Expression of clathrin heavy (CHC) and light (CLC) chains in different Trypanosoma cruzi developmental forms. (A) Immunoblotting of parasite lysates using anti-TcCHC monoclonal IgG (upper bands), TcCLC antiserum (lower bands) and TcActin antiserum (middle bands). Epi: culture epimastigotes; Trypo: metacyclic trypomastigotes; Ama: isolated amastigotes; Vero: Vero cells (amastigote control). Each lane contained 15 μg protein. (B): CHC and CLC expression, as determined by the integrated density of protein bands (normalized to TcActin), analyzed using ImageJ. To evaluate expression in amastigotes, the signal obtained for Vero cells was subtracted from that obtained for the amastigotes. Epi: culture epimastigotes in LIT medium; Trypo: metacyclic trypomastigotes; Ama: amastigotes.