Analysis of TcCLC localization in Trypanosoma cruzi using TcCLC antiserum. Subcellular localization of TcCLC in the various developmental forms of T. cruzi. (A–B): epimastigotes. (C–D): metacyclic trypomastigote. (E–F): isolated amastigote. Note the strong immunolabeling adjacent to the kinetoplast (arrow). Nucleus (arrowhead) and kinetoplast DNA were stained with Hoechst 33342. B, D &F: DIC images of the parasite body. Scale bars = 5 μm.