Cellular viability of bone marrow cells (BMCs) treated with AEPa, as measured by MTT, JC-1, propidium iodide and annexin V assays. a) Cell viability was determined using the MTT assay. Treatment of BMCs maintained in culture after 24, 48, 72 and 96 hours with different concentrations of AEPa, (25, 50 and 100 μg/mL). Data are expressed as mean ± SD of three independent experiments. ANOVA followed by Tukey test, p <0.05. b1-b2) Determination of mitochondrial membrane potential (Δψm) by flow cytometry. Δψm detected by JC-1 in untreated BMCs (b1) or treated cells with 100 μg/mL of AEPa after 96 hours (b2) (results from 10.000 events analyzed). c1 and c2) Determination of apoptosis and/or necrosis analyzed by flow cytometry. c1) Untreated cells. c2) BMCs treated with 100 μg/mL AEPa and maintained in culture after 96 hours. The percentages of apoptotic and necrotic cells are presented in the dot plots and are representative of one of three independent experiments (results from 10.000 events analyzed).