Figure 2
From: Impaired wound healing in mice deficient in a matricellular protein SPARC (osteonectin, BM-40)
Expression of fibronectin, α1(I) collagen and cytokeratin 14 (ck-14) transcripts (left panel) and levels of mRNA expression relative to that of GAPDH set at 100 (right panel) in wounds of wild-type (+/+) and SPARC-null (-/-) mice. Five 6 mm diameter wounds were made on the back of each mouse. Biopsies were collected from 3 wild-type and 3 SPARC-null mice 2, 4, and 6 days after the surgery. Skin from the backs of 2 unwounded wild-type and mutant mice (0 day) was collected and used as controls.