Figure 5

Calcium ionophore and SKF 96365-induced apoptosis of control and Scott B lymphoblasts, assessed by TUNEL and flow cytometry. Control and Scott lymphoblasts were treated with A23187 (200 nM, gray) or SKF 96365 (10 μM, black) for 48 h, and then stained for DNA fragmentation by TUNEL as described in the "Experimental Procedures". Fluorescence shifts observed with A23187 or SKF 96365 treatment were compared to those obtained with non-treated cells (empty). Results were reproduced in at least 3 different experiments.