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Figure 2 | BMC Cell Biology

Figure 2

From: The CXC chemokine cCAF stimulates precocious deposition of ECM molecules by wound fibroblasts, accelerating development of granulation tissue

Figure 2

Levels of TN in the granulation tissue of excision wounds. Paraffin-embedded sections of excision wounds treated every other day with vehicle or 1 μg cCAF were immunostained with an antibody against TN. At 3 days after wounding, control wounds (A) show almost no labeling for TN whereas those treated with cCAF already show substantial labeling for TN (B). (C) Immunoblot analysis of proteins extracted from treated and control tissues reveals that treatment with cCAF results in greatly increased levels of TN within 3 days after wounding, but that by seven days, the amount of TN in the treated wounds had decreased whereas untreated wounds showed levels similar to those of cCAF treated at 3 days. All lanes contain equal amounts of total protein, as measured by the DC protein assay (BioRad).

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