Figure 3

Co-sedimentation of Boi1-PH with vesicles. The two panels show immunoblots, probed with anti-Boi1 antibodies, of supernatant (S) and pellet (P) fractions resulting from centrifugation of a mixture of Boi1-PH and vesicles composed solely of PS (top panel) or composed of a 1:20 (mass:mass) ratio of PIP₂ and PS (bottom panel). For this analysis, prior to centrifugation, aliquots of a cytosolic preparation of yeast extract that contained Boi1-PH were incubated with vesicles in PBS buffer that had the following additional concentrations of KCl: 0 (lanes 1 and 2), 50 (lanes 3 and 4), 100 (lanes 5 and 6), and 200 mM (lanes 7 and 8).