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Figure 7 | BMC Cell Biology

Figure 7

From: Probing the importance and potential roles of the binding of the PH-domain protein Boi1 to acidic phospholipids

Figure 7

Relative concentrations of different versions of Boi1 and Boi1-PH in yeast extracts. Shown are immunoblots, probed with anti-Boi1 antibodies. A). Extracts were from boi1 strain Y1284 expressing the following versions of Boi1 from the indicated plasmids: lane 1 = no Boi1 (pPB1306); lane 2 = w.t. Boi1 (pPB2003), lane 3 = Boi1(KK) (pPB2002), lane 4 = Boi1(TK) (pPB2008), and lane 5 = Boi1(KKTK) (pPB1671). B). Extracts were from strain PY967 expressing the following versions of Boi1-PH from the indicated plasmids: lane 1 = no Boi1-PH (pPB1306), lane 2 = Boi1-PH (pPB1695), lane 3 = Myr-Boi1-PH (pPB1498), lane 4 = Boi1-PH(KK) (pPB1697), lane 5 = Myr-Boi1-PH(KK) (pPB1500), lane 6 = Boi1-PH(TK) (pPB1669), lane 7 = Myr-Boi1-PH(TK) (pPB1667), lane 8 = Boi1-PH(KKTK) (pPB1757), and lane 9 = Myr-Boi1-PH(KKTK) (pPB1661).

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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