Projectin assembles within early Z-bands together with other I-Z-I associated proteins. Immunofluorescence microscopy images representing the staining pattern obtained at 48 hour ASP for several Z-band proteins. Pupae were aged and after dissection, the tissues were spread and processed for immunofluorescence as described in Methods. Corresponding left and right panels represent images from double immunostaining on the same cell. Panel A: projectin; panel B: α-actinin; panel C: projectin; panel D: Z(210); panel E: α-actinin, and panel F: Z(210). All tested Z-band proteins, including projectin, colocalize over discrete bands together with F-actin. Scale bar in A is for panels A through F and represents 10 μm.