Projectin assembles independently from myosin heavy chain. Immunofluorescence microscopy images representing the staining pattern obtained at 36–40 hours and 48 hours ASP for projectin and myosin heavy chain. Panels A and B represent the 36–40 hours ASP stage and panels C and D the 48 hours ASP stage. Panels A and C are stained for projectin and panels B and D for myosin. Myosin staining is still diffuse at 48 hours ASP whereas projectin shows the band pattern already described in Figure 3. Scale bar in B represents 10 μm for panels A through D.