Figure 7
From: Mutations in the IGF-II pathway that confer resistance to lytic reovirus infection
Decreased lytic infection of L-cell clones over expressing the IGF-II gene. Constitutively expressed GAPDH was used to assess loading of RNA in lanes. Survival of lytic infection was determined by infecting 105 L-cells or L-cells expressing the pro-IGF2 transgene with varying concentrations of reovirus type 1 (A) or type 3 (B). The multiplicity of infectious virus particles per cell (MOI) is indicated for each virus serotype. Surviving cells were visualized at 4 (A) or 5 days (B) following infection with gentian violet. Transgene expression was determined by northern blot (C). Experiments were repeated three times and a representative experiment is shown.