Figure 2
From: Intracellular shuttling of a Drosophila APC tumour suppressor homolog
FRAP protocol for the analysis of live Drosophila embryos expressing GFP-E-APC. (a) Sketch of the microscopy and data analysis used to determine the mobility of GFP-E-APC in epithelial cells of live embryos. (b) Consecutive face-on views of a live ~6 hours old embryo (stage 11) expressing GFP-E-APC, with squares marking specific sections of the AJs at cell interfaces; the set of yellow squares in the right-hand image illustrate the cell shape changes that took place during the 5 minutes between the two optical sections shown. White bar in this and subsequent figures, 5 μm.