Figure 3

Analytical gel filtration assays with SMC and SMC mutant proteins. A) Comparison of gel filtration profiles of wild type SMC and SMC mutants (as noted on the profiles) incubated with ScpA and ScpB, same amounts as in Fig 1I). 1B) Silver stained SDS-PAGE with fractions loaded corresponding to elution peaks from gel filtration of SMC Walker A mutant incubated with ScpA and ScpB (corresponding to Fig. 1J).