Glycosaminoglycans (GAGs) Effects on C6 Glioma Adhesion to extracellular matrix (ECM) proteins. (A) Dose dependency of C6 glioma adhesion to ECM-coated wells. Wells were coated with different concentrations of type IV collagen (Col IV), Laminin (Ln) or Fibronectin (Fn). Adhesion assays were performed as described in Methods. Results, expressed in Absorbance at 540 nm, were compared with the adhesion in the absence of ECM. Each point represents the mean of four independent experiments performed in triplicate ± SD. Effects of (B) Heparin, (C) chondroitin sulfate and (D) dextran sulfate on C6 Cells adhesion to ECM proteins. Results were compared with cells seeded on ECM substrate only (controls: 100%). Stars in B and C are equivalent for adhesion on laminin or fibronectin. Each point represents the mean of four independent experiments performed in triplicate ± SD. **p < 0.001, ***p < 0.0001 when compared to controls.