Migration of C6 cells on (A) Type IV Collagen, (B) Fibronectin or (C) Laminin. Wells were coated with 10 μg/ml of each ECM molecule. Cell migration was analyzed at 0, 6 or 9 hour. Quantitative migration measurements were calculated as the increase in the ratio beyond the initial radius of the cell population. CS (chondroitin sulfate), Hep (heparin). Each point represents the mean of three independent experiments performed in triplicate ± SD. ***p < 0.0001 when compared to cells seeded on ECM substrate only (without GAG).