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Table 1 GAGs effects on C6 Glioma detachment

From: Glycosaminoglycans modulate C6 glioma cell adhesion to extracellular matrix components and alter cell proliferation and cell migration

Substrate GAG % Detachment
Type IV Collagen Heparin 0% ± 0.14
  Chondroitin Sulfate 7.5 % ± 0.7
  Dextran Sulfate 0% ± 0.2
Fibronectin Heparin 5% ± 0.9
  Chondroitin Sulfate 4% ± 0.6
  Dextran Sulfate 0% ± 0.2
Laminin Heparin 0% ± 0.9
  Chondroitin Sulfate 0% ± 0.12
  Dextran Sulfate 0% ± 0.5
Culture dish plastic Heparin 7% ± 0,2
  Chondroitin Sulfate 5% ± 0,1
  Dextran Sulfate 2% ± 0,3
BSA Heparin 0% ± 0.1
  Chondroitin Sulfate 5% ± 0,7
  Dextran Sulfate 4% ± 0,4
  1. Wells were coated with 10 μg/ml of type IV collagen, Fibronectin or Laminin. Detachment assays were performed as described. Results were compared with cells seeded on ECM substrate only. Coating with BSA or the culture dish plate was used as controls. Each point represents the mean of three independent experiments performed in triplicate ± SD.