Effects of microtubule depolymerisation on GFP-APC distribution in COS-7 cells. Panels A-C. GFP-APC expressing COS-7 cells were incubated for 60 min in 5 μg/ml Nocodazole. GFP-APC associated with remnant microtubules (arrows) can be found in Nocodazole treated cells. However, junctional GFP-APC (arrowheads) is not associated with microtubules and appears unaffected by Nocodazole treatment. GFP-APC green, tubulin red, panel C is a merged image. Panels D-G. Nocodazole treatment of living cells possessing microtubule-associated and junctional-associated GFP-APC (additional file 10). Microtubule-associated GFP-APC (arrow) disperses over time but junctional GFP-APC puncta (arrowheads) are unaffected.