Figure 4

Additional polarity defects associated with loss or over expression of rad26⁺. A. – D. Prolonged G2 arrest affected the morphology of rad26Δ cells. A. rad26⁺ cdc25.22 (TW1261) and B. to D. rad26Δ cdc25.22 (TW1262) cells were arrested at 37°C for 3 h, fixed and stained with Calcofluor. Bar = 8 μm. E. and F. rad26Δ exacerbated the polarity defects of kin1Δ cells. E. kin1Δ (TE550) and F. kin1Δ rad26Δ were grown in liquid culture, fixed with paraformaldehyde and observed with brightfield. Bar = 10 μm. G. – I. Over expression (OE) of rad26⁺ caused polarity defects. G. rad26⁺ with empty vector (TE236 with pTE102), H. rad26⁺ OE rad26⁺ (TE236 with pTE169) and I. rad3Δ OE rad26⁺ (TE570 with pTE169) cells were grown in promoter-derepressing conditions for 20 hours, fixed with paraformaldehyde and stained with DAPI. The arrow points to a cell with an abnormal number of nuclei. Bar = 10 μm.