Figure 6

Lack of apparent involvement of Rho, Ral, Rac, cdc42, and Ras GTPases in the activation of D-JNK by cadmium and arsenite. S2 cells were incubated in the absence ("-") of dsRNA, or in the presence of 700 μg per well of dsRNA specific to neomycin resistance gene ("Neo"), or 100 μg per well of each of dsRNAs specific to small GTP-binding proteins from Drosophila (Rho1, RhoL, RalA, Rac1, Rac2, cdc42, and Ras) ("All"). Four days later, cells were treated with 200 μM CdCl₂ or 200 μM NaAsO₂ for 2 hours. Cell lysates were immunoprobed with antibodies recognizing P-JNK, P-p38, P-ERK, and Ras (B). Expression of Rho1, RhoL, RalA, Rac1, Rac2, cdc42, and Ras mRNAs was detected by RT-PCR using specific primers as indicated (A). An experiment representative of 3 repetitions is shown.