Figure 1

Isolation of Bovine Mesenteric Lymphatic Endothelial Cells. A/ Evan's blue dye, when injected into the lymph node of bovine mesentery (***), outlined lymphatic vessels (**), but not blood vessels (*). B/ A mixed cell population did not form a monolayer in culture. C/ A mixed cell population of predominantly smooth muscle cells and fibroblasts formed circular patterns in culture. D/ Typical morphology of a cell population predominantly comprising of fibroblasts. E/ Morphology of a cell population predominantly comprising of smooth muscle cells. F/ Morphology of a cell population predominantly comprising of lymphatic endothelial cells. G/ Mixed cultures of cells extracted from lymphatic vessels spontaneously formed lymphatic tube-like structures with ends attached to the walls of the tissue culture dish. H/ Enlargement of the lymphatic tube-like structure seen in "G".