Figure 6

Quantitation of apoptotic cells by Hoechst labeling methods. (Top panel) Epifluorescence microscopy images of nuclei showing H9c2 cells treated with 0, 50 and 100 μM tBHP during 3 and 6 hours. Changes in nuclear morphology characteristic of apoptosis (arrows) were detected in Hoechst 33342-stained cells. Scale bar = 20 μm. (Bottom panel) The numbers of apoptotic nuclei were counted and expressed as the percentage of total cells counted (approximately 200 cells per coverslip). Data represents the mean ± SEM of 5 different experiments (bars indicate standard error). *p < 0.05 vs control.