| Lipid-phase | Inter-phase | TCA | Ethanol | Aqueous |
---|
Zero Incubation | 0.8 | 5 | 6 | 0.02 | 88 |
Full Incubation | 0.3 | 6 | 10 | 0.07 | 85 |
Transform ation | 0.5 ± 0.2 | 12 ± 3 | 21 ± 3 | 0.4 ± 0.2 | 66 ± 6 |
- The lipid-phase is the bottom fraction from the chloroform:methanol extraction of the cells. The inter-phase contains cell debris and proteins, and the TCA fraction contains proteins from precipitation of the aqueous fraction. The ethanol fraction contains carbohydrates from the remaining aqueous fraction in 5 volumes of ice-cold ethanol incubated at -20°C over night. The remaining water-soluble fraction contains unused 14C-glucose.