Figure 5

Effects of growth factors on the reporter-cell assay. C2C12BRA cells were stimulated with 1ng/ml (TGF-β1, TGF-β2 and TGF-β3) or 10 ng/ml (FGF-2 and VEGF) of growth factors and the luciferase activity measured after 15 h. Treatment with 14 pM of rBMP-4 served as a positive control. Background luciferase level in the control sample (no BMP) was subtracted from each of the experimental values. The results are expressed as relative luciferase units (RLU). Each point represents the mean ± SEM of triplicate wells from one representative experiment. Similar results were obtained in two separate experiments.