Figure 4From: The pheromone-induced nuclear accumulation of the Fus3 MAPK in yeast depends on its phosphorylation state and on Dig1 and Dig2Deficient Msg5 phosphatase activity in either the nucleus or cytoplasm confers increased sensitivity to pheromone. Halo tests were performed as described in [83]. (A)Wildtype cells; (B) GAL-MSG5 cells; (C) NES-MSG5M45A/NLS-MSG5M45A cells; (D)NES-MSG5M45A/NLS-MSG5M45AC319A; (E) NES-MSG5M45AC319A/NLS-MSG5M45A cells.Back to article page