Figure 2

Transiently transfected constructs are first expressed as cells exit mitosis. (A) Representative fluorescence microscopy image of IMCD3 cells expressing eGFP (green) two hours post-transfection connected by a cytokinetic bridge stained with acetylated-tubulin (red). (B) Graph of the presence of cytokinetic structures and primary cilia over a post-transfection time course. Error bars represent the standard deviation of two experiments, n = 100 for each. The high percentage of transfected cells possessing cytokinetic structures two hours post-transfection indicates that these cells recently completed mitosis. Bar, 10 μm.