Skip to main content
Figure 5 | BMC Cell Biology

Figure 5

From: Induction of autophagy in rats upon overexpression of wild-type and mutant optineurin gene

Figure 5

Immunostaining of optineurin (OPTN), PSMB5, and LC3 in the eyes of a rat chronic hypertension model. The optic nerve damage in the rats was graded from 1 (no injury) to 5 (active degeneration in the entire optic nerve area). A. Retinal cross sections of grades 1, 2.75, and 5 were immunostained. The grade 2.75 and 5 slides showed stronger OPTN and LC3, but weaker PSMB5, staining, compared with that of grading 1.0 (left panel). Higher magnification of staining was shown in insets. Scale bars, 25 μm. Images (20 of 10× fields) were captured and the intensity of each staining in retinal ganglion cells was quantified. Results (mean ± SEM, averaged from 40 cells total) are presented in the bar graph at the right panel. The staining intensity (relative to no injury control grade 1) for optineurin was 1.0 ± 0.04, 1.22 ± 0.04, and 1.36 ± 0.03, respectively, for grades 1, 2.75, and 5. The corresponding relative intensity for PSMB5 was 1.0 ± 0.03, 0.76 ± 0.04, and 0.66 ± 0.04; and that for LC3 was 1.0 ± 0.04, 1.20 ± 0.04 and 1.26 ± 0.03. B. Bar graphs depict the staining intensity of OPTN, PSMB5, and LC3 in retinal ganglion cells in another set of experiments. The retinal cross sections of grades 1.0, 2.58, 2.75, 3.0, and 5.0 were used. The relative staining intensity (mean ± SEM, averaged from 18–31 cells) for optineurin was 1.0 ± 0.02, 1.30 ± 0.03, and 1.43 ± 0.01, respectively, for grades 1, 2.6-3.0, and 5. The corresponding intensity for PSMB5 was 1.0 ± 0.04, 0.88 ± 0.02, and 0.86 ± 0.01; and that for LC3 was 1.0 ± 0.02, 1.13 ± 0.04, and 1.38 ± 0.05. **, P < 0.002; *, P < 0.032 compared to grade 1.

Back to article page