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Fig. 2 | BMC Cell Biology

Fig. 2

From: Signal dependent ER export of lemur tyrosine kinase 2

Fig. 2

Mutation of the di-acidic export motif causes retention of LMTK2 within the ER. a HeLa cells stably expressing shRNA against LMTK2 were transiently transfected with siRNA resistant constructs for either WT LMTK2 or A1110SA LMTK2. b Following immunofluorescence labeling, LMTK2 was visualized with secondary antibodies conjugated to Alexa 488 dyes (green) and calreticulin observed as the transiently expressed mRFP fusion protein (red). Nuclei are stained blue using DAPI. Bar = 10 μm. c Pearson’s coefficients were determined for HeLa cells by imaging at least 30 cells from three independent experiments, encompassing randomly selected fields of view. *p < 0.01 for difference by Student’s t-test

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