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Fig. 3 | BMC Cell Biology

Fig. 3

From: Signal dependent ER export of lemur tyrosine kinase 2

Fig. 3

Gradient fractionation of cellular organelles shows a retention of A1110SA LMTK2 within the ER. HeLa cells expressing either WT LMTK2 of A1110SA LMTK2 were subject to homogenization in a detergent free buffer, using a Dounce-type homogenizer. Post-nuclear supernatants were applied to an Optiprep™ gradient and resolved by centrifugation. a Fractions were collected and proteins resolved by SDS-PAGE on a 4–12 % gradient gel prior to immunoblotting. b Comparison of relative band intensities of LMTK2 in each fraction (mean data from 4 separate experiments)

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