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Fig. 5 | BMC Cell Biology

Fig. 5

From: Signal dependent ER export of lemur tyrosine kinase 2

Fig. 5

Effect of ER export motif mutations on protein half-life. a Cells expressing either WT or A1110SA LMTK2 were subject to labeling with 35S Met/Cys to steady-state. Cells were switched to 35S-free media and sampled at various time-points. LMTK2 was subject to immunoprecipitation and resolved by SDS-PAGE. A typical gel is shown. b Analysis of three separate experiments yields half-lives of 2.5 h for WT LMTK2 () and 3.5 h for A1110SA LMTK2 (▲) (p < 0.05 for difference between constructs)

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