Fig. 1

HAF-4- and HAF-9-localizing intestinal organelles are distinct from lipid droplets. a and b Comparison of the localization of HAF-9::mCherry with the lipid droplet markers DHS-3::GFP (a) and ATGL-1::GFP (b) in Is[dhs-3::GFP]/Is[haf-9::mCherry] and Is[atgl-1::GFP];Is[haf-9::mCherry], respectively. DHS-3::GFP or ATGL-1::GFP (green), HAF-9::mCherry (magenta), the merged images, and the corresponding DIC images are presented. DHS-3::GFP and HAF-9::mCherry did not localize to the surface of identical intestinal granules. c Nile Red staining of the wild-type N2 (left) and the haf-4(ok1042) haf-9(gk23) double-mutant worms (right). Nile Red (magenta) and the merged images with DIC are presented. Nile Red-positive intestinal granules were intact even in the absence of the HAF-4- and HAF-9-localizing organelles. d Expression of the lipid droplet marker DHS-3::GFP in the wild-type (top left), haf-4(ok1042) (top right), haf-4(gk240) (bottom left), and haf-9(gk23) (bottom right) worms. DHS-3::GFP-positive lipid droplets did not decrease in these mutants. Bars, 5 μm