Fig. 3

HAF-4- and HAF-9-localizing intestinal organelles are not acidified gut granules. a Comparison of the localization of HAF-9::mCherry with GLO-1::GFP in Is[ges-1p::glo-1::GFP];Is[haf-9::mCherry]. GLO-1::GFP (green), HAF-9::mCherry (magenta), the merged image, and the corresponding DIC image are presented. GLO-1::GFP did not localize to the mCherry-edged intestinal granules (arrows). Arrowheads indicate the accumulation of mCherry in the gut granules. b The localization of HAF-4::GFP (top) and HAF-9::mCherry (bottom) in the wild-type (left) and glo-1(zu391) (right) worms. The corresponding DIC images are also presented. HAF-4::GFP-positive and HAF-9::mCherry-positive intestinal granules were observed even in the mutant worms. Bars, 5 μm