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Fig. 4 | BMC Cell Biology

Fig. 4

From: Characterization of HAF-4- and HAF-9-localizing organelles as distinct organelles in Caenorhabditis elegans intestinal cells

Fig. 4

HAF-4- and HAF-9-localizing organelles are associated with the endocytic pathway. a Co-localization of GFP::RAB-7 and HAF-9::mCherry in Is[haf-9::mCherry];Ex[GFP::rab-7]. GFP::RAB-7 (green) HAF-9::mCherry (magenta), the merged image, and the corresponding DIC image are presented. b Localization of GFP::RAB-7 in the wild-type (top left) haf-4(ok1042) (middle left), haf-4(gk240) (bottom left), haf-9(gk23) (top right), and lmp-1(nr2045) (middle right). GFP::RAB-7 (green) and the corresponding DIC images are presented. In the haf-4 and haf-9 mutants, exhibiting defects in the HAF-4- and HAF-9-localizing organelles, the number of intestinal granules with GFP fluorescence on their surface decreased. In lmp-1(nr2045), GFP fluorescence was detected on the surface of the enlarged vacuole (asterisks). c Subcellular localization of HAF-4::GFP (left) and HAF-9::mCherry (right) in the wild-type (top) and rab-7(ok511) (bottom). The corresponding DIC images are also presented. HAF-4::GFP-positive and HAF-9::mCherry-positive intestinal granules dramatically decreased in the rab-7 mutant. Bars, 5 μm

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