Fig. 6
SiRNA transfer through TTs between different Cx-expressing HeLa cells. a A pair of Cx45-expressing HeLa cells connected through the TT. b SiRNA/AF488 (2 μM) introduced into the patch pipette entered the cell-1 after patch opening, spread along the TT but did not enter the remote cell-2. c and d Kinetics of siRNA/AF488 accumulation in the cell-1 and the cell-2, respectively. gT measured at the end of the experiment was 2.6 nS. Arrows point to the moments of patch opening in the cell-1 and the cell-2. e and f Kinetics of AF488 accumulation in the cell-1 and the cell-2, respectively. g A pair of Cx40-CFP-expressing HeLa cells connected through the TT. h After the patch was opened in the cell-1, siRNA/AF488 entered the cell-1, spread along the TT, and accumulated in the cell-2. i and j Kinetics of siRNA/AF488 accumulation in the cell-1 and the cell-2, respectively. siRNA/AF488 accumulation in the cell-2 was arrested by octanol (0.5 mM), a GJ blocker. gT measured at the end of the experiment was 10 nS