Fig. 2

Gap junction plaques (arrows) and annular gap junction vesicles (arrowheads) shown with transmission electron (a), freeze fracture electron (b), and immunofluorescence microscopy (c). Staining cortical actin (green in c) helps to define the cell borders. The protoplasmic (P) and extracellular (E) fracture faces have been labeled in the replica of the gap junction plaque (in b). Nucleus = n. Bars: 100 nm in (a), 60 nm in (b), and 10 μm in (c). (a from ref. [58] and b from ref. [206])