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Fig. 3 | BMC Cell Biology

Fig. 3

From: Molecular mechanisms regulating formation, trafficking and processing of annular gap junctions

Fig. 3

Immunocytochemistry (a) and 3D-volume reconstructions (b-d) demonstrating in detail the association of clathrin (red) with a Cx43 (green) gap junction plaque. An area of the gap junction plaque can be appreciated in the rotated views as a bud that likely has not yet detached from the plaque (a-d arrowheads). It can be confirmed in the rotated views that an annular gap junction is intimately associated with clathrin (arrows a-d). A rotation around the Y-axis in the 3D-reconstruction images allows more information to be obtained on the morphology of the gap junction structures as well as their relationship to clathrin. The images seen in (b) and (c) (arrows) have been enlarged (b'-c'). The 3D-reconstruction was rendered with the Amira 3D Software for Life Sciences, FEI™ (Hillsboro, Oregon). Bar: 5 μm in (a-d), 1.5 μm in (b’, c’)

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