Fig. 6
From: Molecular mechanisms regulating formation, trafficking and processing of annular gap junctions
Time-lapse image sequence of cells expressing Cx43-GFP. As evident from the phase/fluorescence overlay image (a) one of the two contacting cells is expressing RFP-tagged clathrin (red). In the corresponding montage of these cells, the gap junction plaque invaginates to form a gap junction bud (arrow), which is then scissored from the membrane to produce an annular gap junction vesicle (arrowhead) (b, 1-6). Time is in hours:minutes:seconds. Bar: 15 μm. (From ref. [58])