Fig. 5
From: Early changes in the metabolic profile of activated CD8+ T cells
CTLA4-/- OT-I T cells showed no decrease in lactate production and IL-2 production and CTLA-4 mediated reduction of surface CD25 expression in WT CD8 T cells. Purified CD8+ T cells from CTLA-/- or CTL4+/+ mice were stimulated with OT-I tetramers for indicated time points, cells were analyzed for intracellular lactate concentrations (a), intracellular IL-2 concentrations by ELISA (b), and (c) Naïve (CD62Lhigh) CD8+ T cells from WT mice were stimulated with anti-CD3/CD28/CTLA-4 or anti-CD3/CD28/Isotype control-coupled microspheres. Surface expression of CD25 was detected by flow cytometry. Geomean numbers of CD25 fluorescence are depicted for the respective conditions