Fig. 6

Inhibition of AKT abrogates lactate production. Purified CD8⁺ T cells were treated with OT-I tetramers for the indicated time periods. Samples were analyzed for production of lactate in presence of the AKT-Inhibitor AKT 8 and the mTOR-inhibitor Rapamycin (a). Samples were analyzed for upregulation of LDH by Western blotting in the presence of 3 different AKT inhibitors (b). Expression of GLUT1 after tetramer stimulation in presence and absence of AKT inhibitor was analyzed via FACS (c) and calculated from 3 independent experiments (d). Glucose uptake was analyzed 24 h after stimulation of CD8⁺ T cells with tetramers in the absence and presence of AKT inhibitor and apigenine (e) and calculated from 4 independent experiments (f)