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Fig. 2 | BMC Cell Biology

Fig. 2

From: Loss of hif-1 promotes resistance to the exogenous mitochondrial stressor ethidium bromide in Caenorhabditis elegans

Fig. 2

hif-1 and djr-1.1 mutants require the nsy-1/sek-1/pmk-1 signalling pathway for EtBr resistance. a pmk-1 is required by hif-1 and djr-1.1 for growth on EtBr containing plates. The first generation of various worm strains containing single or double mutant alleles as listed were grown on worm plates containing nematode growth medium and 50 μg/ml of EtBr, and the percentage of animals that reached the adult stage were determined. Two single mutants of hif-1, hif-1(ia4) and hif-1(ia7), demonstrated growth in the presence of EtBr. All subsequent experiments were done with the hif-1(ia4) allele. Furthermore, it was found that the loss of pmk-1 abrogates EtBr resistance of hif-1 and djr-1.1 mutants. Asterisk marks denote significant difference between EtBr-treated N2 and EtBr-treated mutants (* denotes p < 0.05 ** p < 0.01, *** p < 0.001). One-way Anova was performed to test for the statistical significant difference in growth between strains. b pmk-1 is induced in the presence of EtBr in wild type, hif-1, djr-1.1 and vhl-1 mutants. Transcript levels of two pmk-1 targets (F08G5.6 and F35E12.5) in control (0 μg/mL, “control” open box) and EtBr-treated (50 μg/mL, “ + EtBr”; grey box) samples were measured using qRT-PCR. # indicates not determined, as vhl-1 mutant and pmk-1 double mutants were unable to survive on worm plates containing EtBr. Results are from three independent experiments

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