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Table 1 Details of the primer sequences and cycling conditions used for RT-PCR

From: Variation in human dental pulp stem cell ageing profiles reflect contrasting proliferative and regenerative capabilities

Gene marker Primer sequence Annealing temp. (°C) Cycles NCBI reference sequence
CD73 F:5′GTCGCGAACTTGCGCCTGGCCGCCAAG-3′
R: 5′-TGCAGCGGCTGGCGTTGACGCACTTGC-3′
65 35 NM_001204813.1
CD90 F:5′- ATGAACCTGGCCATCAGCATCG-3′
R:5′- CACGAGGTGTTCTGAGCCAGCA-3′
55 35 NM_006288.3
CD105 F:5′-GAAACAGTCCATTGTGACCTTCAG-3′
R: 5′-GATGGCAGCTCTGTGGTGTTGACC-3′
65 35 NM_001114753.2
CD271 F:5′- CTGCAAGCAGAACAAGCAAG-3′
R:5′- GGCCTCATGGGTAAAGGAGT-3′
55 35 NM_002507.3
CD45 F:5′-GTGACCCCTTACCTACTCACACCACTG-3′
R:5′-TAAGGTAGGCATCTGAGGTGTTCGCTG-3′
65 35 NM_002838.4
hTERT F:5′- CGGAAGAGTGTCTGGAGCAA-3′
R:5′- GGATGAAGCGGAGTCTGGA-3′
55 40 NM_198253.2
p53 F:5′- AGACCGGCGCACAGAGGAAG-3′
R:5′- CTTTTTGGACTTCAGGTGGC-3′
55 35 NM_001126118.1
p21waf1 F:5′- GGATGTCCGTCAGAACCCAT-3′
R:5′- CCCTCCAGTGGTGTCTCGGTG-3′
60 35 NM_001291549.1
p16INK4A F:5′- CTTCCTGGACACGCTGGT-3′
R:5′- GCATGGTTACTGCCTCTGGT-3′
55 35 NM_001195132.1
OCN F:5′-GCAGGTGCGAAGCCCAGCGGTGCAGAG-3′
R: 5′-GGGCTGGGAGGTCAGGGCAAGGGCAAG-3′
62 35 NM_199173.4
OPN F:5′- ATCACCTGTGCCATACCA-3′
R:5′- CATCTTCATCATCCATATCATCCA-3′
55 35 NM_001251830.1
PPARγ F:5′-GCCATCAGGTTTGGGCGGATGCCACAG-3′
R: 5′-CCTGCACAGCCTCCACGGAGCGAAACT-3′
62 35 NM_138711.3
LPL F:5′-GCTGGCATTGCAGGAAGTCTGACCAATAA-3′
R:5′-GGCCACGGTGCCATACAGAGAAATCTCAA-3′
55 35 NM_000237.2
β-actin F:5′-AGGGCAGTGATCTCCTTCTGCATCCT-3′
R:5′- CCACACTGTGCCCATCTACGAGGGGT-3′
65 35 NM_001101.3