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Fig. 2 | BMC Cell Biology

Fig. 2

From: Hinokitiol induces DNA demethylation via DNMT1 and UHRF1 inhibition in colon cancer cells

Fig. 2

Hinokitiol decreases DNMT1 mRNA and protein expression in HCT-116 cells. The level of DNMT1 mRNA was determined in HCT-116 and SW480 cells using RT-PCR (a) and quantitative real-time PCR (qRT-PCR) (b). PCR products were gel-run in 2% agarose and visualized in ultraviolet (UV). β-actin was used as a quantitative control. The quantitative DNMT1 mRNA level was measured using qRT-PCR after hinokitiol treatment at indicated concentrations and times in HCT-116 cells (c and d). Total protein was isolated from cells treated with indicated concentrations of hinokitiol for 72 h and western blotting was performed to detect DNMT1 expression (e). The results were representative of three independent experiments. Cells treated with DMSO and 5-aza-dC were used as negative and positive controls, respectively. The β-actin was used as a loading control. * indicates a significant difference at the level of < 0.05. M, 100 bp DNA ladder

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