Fig. 1

Expression of MEF2 and ACTN2 under different concentrations of HGF. Real-time PCR of MSC and myoblast (Mb) mono- and co-cultures under HGF stimulation as well as in unstimulated controls. Expressions are demonstrated in x-fold difference compared with unstimulated cells cultivated in basic differentiation medium (control = 1) using the 2^-ΔΔCt method. Markers are presented with mean +/- SD. a Significant and highly significant higher expression of MEF2 in co-cultures after 2 d compared with 7 d using 10, 30 and 60 ng/ml HGF. b In co-cultures, ACTN2 expression was upregulated during early stimulation compared with unstimulated control groups. 100 ng/ml HGF over 7 d induced the strongest ACTN2 expression. c Strongest MEF2 expression in MSC monocultures could be achieved with 10 ng/ml HGF after stimulation for over 7 d. d Seven-day stimulation with 10, 60 and 100 ng/ml HGF induced almost equal or higher levels of ACTN2 in MSC monocultures compared with 2 d stimulation. e-f In Mb, a dose-dependent increase in MEF2 (e) and ACTN2 (d) expression was demonstrated from 10 to 60 ng/ml HGF during early stimulation. Increased levels of MEF2 and ACTN2 under HGF during early stimulation in co-cultures and Mb monocultures compared with unstimulated controls. Mb of three different isolations were used in three independent experiments. Three replicates of each were used. (** = p ≤ 0.01). (* = p ≤ 0.05)