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Fig. 2 | BMC Cell Biology

Fig. 2

From: Mesenchymal stem cells and myoblast differentiation under HGF and IGF-1 stimulation for 3D skeletal muscle tissue engineering

Fig. 2

Expression of MEF2 and ACTN2 under different concentrations of IGF-1. Real-time PCR of MSC and myoblast (Mb) mono- and co-cultures under IGF-1 stimulation as well as in unstimulated controls. Expressions are demonstrated in x-fold difference compared with unstimulated cells cultivated in basic differentiation medium (control = 1) using the 2-ΔΔCt method. Markers are presented with mean +/- SD. a Overall higher expressions of MEF2 in co-cultures under the different IGF-1 concentrations compared with unstimulated conditions after 2 d. Significant and highly significant higher levels of MEF2 after 2 d compared with 7 d using 10 and 30 ng/ml IGF-1. b Overall higher expressions of ACTN2 in co-cultures under the different IGF-1 concentrations compared with unstimulated conditions after 2 d. c Stimulation with 60 ng/ml IGF-1 over 2 d induced the strongest upregulation of MEF2 in MSC monocultures. d Overall increased ACTN2 expression in MSC monocultures was observed during early stimulation, with highest levels under 10 ng/ml IGF-1. e-f Early stimulation with IGF-1 induced higher MEF2 (e) and ACTN2 (f) expressions in Mb monocultures compared with controls, with strongest expression under 30 ng/ml IGF-1. Increased MEF2 expression in co-cultures and Mb monocultures after 2 d compared with unstimulated controls. The highest MEF2 and ACTN2 levels were detected in Mb monocultures. Mb of three different isolations were used in three independent experiments. Three replicates of each were used. (** = p ≤ 0.01). (* = p ≤ 0.05)

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