Fig. 3
Expression of myogenic differentiation markers and IGFBPs under the influence of HGF and IGF-1. Real-time PCR of MSC and myoblast (Mb) mono- and co-cultures stimulated with HGF + IGF-1, HGF, IGF-1 or cultivated in unstimulated controls. Expressions are demonstrated in x-fold difference compared with Mb (=1) using the 2-ΔΔCt method. Markers are presented with mean +/- SD. a After 2 d, the strongest DES upregulation was demonstrated in unstimulated controls. Throughout all conditions in MSCs, much higher levels of DES compared with co-cultures and Mb were observed. b After 14 d, strongest MYOG expression was detected under HGF stimulation compared with control myoblasts. MYOG could only be detected in one out of three experiments. c The highest expression of ACTN2 was observed in IGF-1 stimulated groups, both in co-cultures and MSC monocultures. d The strongest upregulation of MyHC2 in co-cultures was observed under IGF-1 stimulation. In MSC monocultures, the levels of MyHC2 remained under all conditions lower than Mb. e In co-cultures, the highest IGFBP4 levels were observed under IGF-1 stimulation. In MSC monocultures, HGF induced the strongest upregulation of IGFBP4. f In co-cultures, the highest IGFBP5 levels were observed under IGF-1 stimulation. In MSC monocultures, the expression of IGFBP5 remained lower under all conditions compared with Mb. g In both cell groups, the levels of IGFBP6 were overall lower than in control Mb. Mb of three different isolations were used in three independent experiments. Three replicates of each were used