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Fig. 5 | BMC Cell Biology

Fig. 5

From: Mesenchymal stem cells and myoblast differentiation under HGF and IGF-1 stimulation for 3D skeletal muscle tissue engineering

Fig. 5

Fluorescence microscopy of MEF2 in co-cultures after 14 d. A positive staining of MEF2, a co-transcriptional factor expressed during muscle differentiation, in MSC and Mb co-cultures in control groups without HGF/IGF-1 after 14 d. Merge of DAPI (blue, nuclear staining), GFP (green, transduced MSC) and MEF2 (red, with Alexa fluor 594 as secondary antibody). The red fluorescence demonstrates the characteristic perinuclear localisation of MEF2. Scale bars represent 50 μm. Magnification 200x

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