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Fig. 1 | BMC Cell Biology

Fig. 1

From: Loss of pericyte smoothened activity in mice with genetic deficiency of leptin

Fig. 1

Genetic Leptin Deficiency Does Not Alter Leptin Receptor Expression But Suppresses Hh Activity in HSCs. a. Primary HSCs were isolated from WT mice or ob/ob mice, and cultured on plastic dishes in serum-containing medium for 2 h (d0) or 7 days (d7). FACS analysis of (left) Leptin and (right) Leptin receptor (OBRa plus OBRb) immunoreactivity in quiescent (day 0, d0) and myofibroblastic (culture day 7, d7) HSCs. Red lines indicate Isotype controls. b. FACS analysis analysis of Hh target gene products (Ptc, Gli1, Gli2) in quiescent and myofibroblastic HSC from WT and ob/ob mice. Red lines indicate Isotype controls

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