Fig. 3

SING formation required the nuclear import pathway. a RNAi of ima-1, ran-1, and arx-5 reduces SING formation. Worms expressing GFP::Ub in the germline were soaked in 500 mM NaCl for 60 min. Proximal oocytes are shown. Control worms treated with vector RNAi formed SINGS normally. Scale bar indicates 10 μm. b The percentage of oocytes forming SINGs after treatment as described in A. Oocytes were scored for both GFP::Ub and RPT-1::mCh at SINGs. A total of 1020 oocytes were analyzed for each condition from 3 independent experiments (n = 30 worms). Statistical significance was calculated by a two-tailed z test: **p < 0.01 and ***p < 0.001. c RNAi of smo-1 reduces SING formation in a worm strain expressing GFP::Ub in the germline. Proximal oocytes are shown. Scale bar indicates 10 μm. d The percentage of oocytes forming SINGs after treatment as described in C. A total of 1020 oocytes were analyzed from 3 independent experiments (n = 30 worms). Statistical significance was calculated by a two-tailed z test: ***p < 0.001