Skip to main content
Fig. 6 | BMC Cell Biology

Fig. 6

From: Stress induced nuclear granules form in response to accumulation of misfolded proteins in Caenorhabditis elegans

Fig. 6

Oxidative stress and Starvation induce SING formation. a Oxidative stress and starvation in gonads. Worms expressing GFP::Ub and mCh::H2B were grown on bacteria with control or ubc-18 RNAi and subjected to 10 mM H2O2 for 30 min or starvation for 48 h. Distal gonads are shown. Oxidative stress and starvation induced SING formation in vector treated worms. SINGs did not form in ubc-18 worms soaked in H2O2 or starved. Scale bar indicates 10 μm. b Quantification of the percentage of oocytes forming SINGs after treatment as described in A. A total of 250 oocytes were collected from 3 independent experiments (n = 15 worms). Statistical significance was calculated by a two-tailed z test: ****p < 0.0001. c Heat shock does not induce SINGs. Worms expressing GFP::Ub and mCh::H2B were subjected to heat shock by placing them at 37 °C for one hour. In control worms incubated at 25 °C for 1 h, 0/600 oocytes showed SINGs. In the heat shock group, 0/600 oocytes showed SINGs. Data were collected from 2 independent experiments (n = 20 worms). Scale bar indicates 10 μm

Back to article page