Fig. 9

SINGs contain the TIAR-2 protein. a Worms expressing GFP::TIAR-2 were soaked in M9 or 500 mM NaCl for 60 min and then stained with an antibody to the 19S proteasome. The merged image shows the TIAR-2, proteasome, and DAPI channels. In M9 treated worms, few oocytes showed nuclei with TIAR-2 and proteasome colocalization (38/600). Whereas, in salt stressed worms, the number of TIAR-2 and proteasome colocalization events increased (212/600 oocytes). Data were collected from 3 independent experiments (n = 30 worms). Scale bar indicates 10 μm. b Day 4 adults expressing TIAR-2::GFP were soaked in a watch glass containing 500 mM NaCl with or without MG132 for 60 min. Worms that were not treated with MG132 showed TIAR-2 in SINGs, whereas MG132 treated worms showed fewer TIAR-2 containing SINGs. c Quantification of the percentage of TIAR-2 in SINGs as described in B. A total of 90 proximal oocytes were observed from 3 independent experiments (n = 30 worms). Statistical significance was calculated by a Fisher’s Exact test: ****p < 0.0001