Fig. 6

Osteogenic differentiation on Ti disks. a-c SEM images of the different cells types differentiating on Ti alloy disks. (a) DPPSC, (b) Bone-like DPPSC, (c) SAOS-2. Stars indicate the Ti surface without cells. Scale Bars: 40 μm (a1, b1, c1), 10 μm (a2, b2, c2). d ALP activity (U/L) of TI DPPSC, TI B.DPPSC and TI SAOS-2 differentiating on Ti alloy disks at week 1 and 2 (n = 3). e-g RT-PCR (e) and qRT-PCR (f, g) gene expression analysis of differentiation (RUNX2, COL1 and OC) and adhesion markers (ITGα3 and ITGαV) at second week of cell differentiations on Ti alloy disks (n = 3). TI SAOS-2 was used for normalization. DPPSC at day 0 of differentiation were used as a negative control and GAPDH was used as housekeeping gene. *P < 0.05